Insights
Notes from the design loop
Working notes on the parts of aptamer discovery that rarely make it into a paper: why enrichment is not optimization, which constraints have to be set before generation begins, and what a failed binding assay is actually telling you.
Learning from the misses: post-assay error analysis in a closed design loop
A candidate that shows no binding is three different results wearing the same coat. Separating them is most of the work.
Developability first: the constraints we set before generating a single aptamer
Nuclease resistance, clearance, immunostimulation and synthesis cost are design inputs, not downstream repairs.
Why SELEX gets stuck: enrichment is not optimization
Selection rewards whatever survives capture and amplification. That is not the same objective as the one your program cares about.
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